The AlphaLISA® immunoassay kit for human CXCL12 (SDF-1) enables the quantitative determination of human CXCL12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
CXCL12 is a small protein of 89 amino acids also known as Stromal-Derived Factor (SDF-1). It is a member of the CXC family of chemokines. CXCL12 is a strong chemoattractor for lymphocytes and is involved in angiogenesis in both fetal and adult organisms by recruiting lymphocytes to newly formed vessels. It also plays a role in bone morphology by inhibiting bone formation by osteoclast. Finally, as a natural ligand for the receptor CXCL4, it inhibits binding of HIV1 and 2 to the receptor, thus being a natural anti-AIDS compound.
|Assay Target||CXCL12, SDF1|
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual explains how to run the AlphaLISA human CXCL12 detection assay.