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Mature C-X-C Motif Chemokine 11 (CXCL11), also known as Interferon Inducible T-Cell alpha Chemoattractant (I-TAC), is a small chemokine of 73 amino acids with a molecular weight of 8.3 kDa. CXCL11 is one of the most potent chemoattractants for activated T cells and is a key player in the regulation of the immune response. It is also known to attract monocytes, B cells, Th1 lymphocytes and NK cells. CXCL11 mediates its biological actions through binding to the CXCR3 and CXCR7 receptors. CXCL11 is highly expressed in blood leukocytes, pancreas, and liver tissues and is produced, to a lower extent, by the thymus, the spleen, and the lung. Its production is induced by IFN?, and it possesses anti-microbial activity against many bacteria. CXCL11 is expressed in a variety of skin disorders, including allergic contact dermatitis, lichen planus, and mycosis fungoides.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.