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Cortisol (hydrocortisone) is a steroid derived from cholesterol. Cortisol can be found in the blood as free or bound to cortisol-binding globulins, such as transcortin. Cortisol levels follow a diurnal pattern, typically highest in the morning upon wake up and lowest just before sleep. Cortisol levels have been linked to metabolism regulation, glucogenesis promotion, liver glycogen deposition, and depression of glucose burn. Cortisol levels have also been positively correlated to stress and increasing blood pressure.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In this AlphaLISA competition assay, a biotinylated cortisol tracer binds to the Streptavidin-coated Donor beads and anti-cortisol antibody associated to AlphaLISA Acceptor beads. When no cortisol is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. With increasing concentrations of endogenous cortisol from the sample, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.