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Cortisol (human) AlphaLISA Detection Kit, 500 Assay Points

AlphaLISA no-wash immunoassay kit for detection of human cortisol in buffer or serum.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
100 assay points
976.00 USD
500 assay points
2133.00 USD
5,000 assay points
14200.00 USD
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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cortisol (hydrocortisone) is a steroid derived from cholesterol. Cortisol can be found in the blood as free or bound to cortisol-binding globulins, such as transcortin. Cortisol levels follow a diurnal pattern, typically highest in the morning upon wake up and lowest just before sleep. Cortisol levels have been linked to metabolism regulation, glucogenesis promotion, liver glycogen deposition, and depression of glucose burn. Cortisol levels have also been positively correlated to stress and increasing blood pressure.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In this AlphaLISA competition assay, a biotinylated cortisol tracer binds to the Streptavidin-coated Donor beads and anti-cortisol antibody associated to AlphaLISA Acceptor beads. When no cortisol is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. With increasing concentrations of endogenous cortisol from the sample, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.


Assay Target Cortisol
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Format Microplates
Product Brand Name AlphaLISA
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 500 assay points
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