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Corticosterone (CORT) is a member of corticosteroids and is also known as 17-deoxycortisol or 11β 21-dihydroprogesterone. CORT is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone and is the precursor of pregnenolone, cortisol, and aldosterone. CORT production has been shown to increase with stress and the elevated CORT levels have been associated with impairment of long term memory retrieval. In some species, CORT is a main glucocorticoid, involved in regulation of energy, immune reactions, and stress responses. Furthermore, CORT serves as a major homeostatic modulator of sodium and potassium levels in vivo.
AlphaLISA technology allows the detection of molecules of interest in buffer and in serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. Biotinylated corticosterone-BSA complex and free corticosterone (in standards or in samples) compete for binding to anti-corticosterone antibody that is conjugated to AlphaLISA Acceptor beads. In the presence of Streptavidin-coated Alpha Donor beads, biotinylated Corticosterone-BSA complex binds to the Streptavidin-coated Alpha Donor beads. When corticosterone-BSA binds to anti-corticosterone AlphaLISA acceptor beads, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.