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AlphaLISA Human cIAP1 BIR3 Binding Kit, 500 Assay Points

The AlphaLISA® Human cIAP1 BIR3 Binding Kit is designed to screen and characterize compounds that specifically bind the E3 ligase, cIAP1 BIR3 domain, using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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AL3167C
500 Assay Points
2977.00 USD
 
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AL3167F
5,000 Assay Points
14600.00 USD
 
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Overview

cIAP1, also called BIRC2, is a member of the Inhibitor of Apoptosis Proteins (IAP) family. IAP proteins are involved in multiple biological processes, such as innate immunity, and play an important role in apoptosis inhibition. cIAP1 overexpression has been associated with cancer resistance, making it an attractive target in cancer therapy where several therapeutic strategies have been investigated, such as SMAC mimetics.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Points (200-50,000) 500 Assay Points
Assay Target clAP1 BIR3
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Autoimmunity, Neuroscience, Oncology
Unit Size 500 Assay Points
Resources, Events & More
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Application Brief

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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

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Poster

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