AlphaLISA Human Cereblon Binding Kit, 5,000 Assay Points

Cereblon (CRBN) is a ubiquitously expressed protein which functions as a substrate recognition subunit within the CRL4-CRBN E3 ubiquitin ligase complex and targets specific proteins for proteolysis through the ubiquitin proteasome system (UPS). Cereblon plays a key role in normal limb outgrowth, normal cell proliferation and metabolism, and in memory and learning. Cereblon is also the primary direct target of the immunomodulatory drugs Thalidomide, Pomalidomide, and Lenalidomide, which are FDA approved for treating multiple myeloma. Like other E3 ligases such as VHL, Cereblon E3 ligase property is commonly used in targeted protein degradation strategy, eg PROTAC.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume

AlphaLISA technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

This product is also available using HTRF Technology. To learn more, visit HTRF Cereblon Binding Kit webpage.

PerkinElmer has made available a range of documents with educational content and reagents dedicated to PROTAC to help you in your research. Visit our dedicated webpage to learn more.