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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Cluster of differentiation 70 (CD70), also known as CD27L or TNFSF7, is a type II transmembrane receptor protein expressed on activated T and B cells. It binds to CD27 on mature T-cells and a subset of thymocytes. Ligation of CD70 results in the proliferation of costimulated T cells. CD70 expression has been shown to be upregulated in range of lymphomas. Treatment with anti-CD70 antibodies has been introduced as a possible treatment for CD70+ tumors.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual describes how to run the AlphaLISA human CD70 assay kit.