The AlphaLISA® human CD28/CD86 binding kit is designed for the detection of binding activity between human CD28 and CD86, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
true falseFor research use only. Not for use in diagnostic procedures.
AlphaLISA features:
AlphaLISA® technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
The AlphaLISA human CD28/CD86 binding kit allows determination of cross-reactivity of human anti-CD28 or CD86 reagents with cynomolgus proteins, as well as development or screening of novel human CD28 and CD86 reagents that block the binding of this interaction. Using the AlphaLISA human CD28/CD86 binding assay, small molecule antagonists or large molecule therapeutics and blocking antibodies can be screened in microplate format.
Assay Target | CD28, CD86 |
---|---|
Assay Target Class | Protein |
Assay Technology | Alpha |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA |
Shipping Condition | Blue Ice |
Therapeutic Area | Immuno-oncology |
Unit Size | 500 Assay Points |
Many tumor cells, which under normal, healthy conditions would be recognized by the body’s T cells and thereby targeted for destruction, have developed ways to evade the host immune system by taking advantage of immune checkpoint pathways. Among the most promising approaches to activating therapeuti ...
Immune checkpoints serve a critical role in the immune system to prevent autoimmunity and manage the degree and duration of an immune response. Cytotoxic T-Lymphocyte-associated protein 4 (CTLA-4 or CD152) is an inhibitory transmembrane protein involved in an immune checkpoint of significant inte ...
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...
Chimeric antigen receptor (CAR) T-cell therapy has transformed the field of immuno-oncology providing a novel approach to treating certain blood cancers. As innovation progresses with the design of next-generation chimeric receptors and a better understanding of the tumor microenvironment, CAR-T ...