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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
CD117, also known as stem cell growth factor receptor or proto-oncogene c-kit, is a cytokine receptor encoded by the KIT gene. This receptor is found on the surface of hematopoietic stem cells and binds to stem cell factor, which then forms a dimer that activates its intrinsic tyrosine kinase activity. CD117 plays an important role in cell survival, proliferation and differentiation. Levels and mutations of CD117 are related to many diseases such as melanoma, leukemia, hematopoietic progenitors, and mast cell disease. This AlphaLISA detection kit allows for the detection of CD117 levels in serum, plasma, and cell culture supernatants.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
This manual explains how to run the AlphaLISA no-wash human CD117 detection assay.