The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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C-C Motif Chemokine 5 (CCL5), also known as Regulated upon Activation, Normal T cell Expressed and Secreted (RANTES), is a 7.8 kDa protein that plays a primary role in the inflammatory immune response. CCL5 binds to C-C chemokine receptor (CCR) types 1, 3, and 5. CCL5 dimerization and oligomerization, as well as binding to glycosaminoglycan, are key elements of cell recruitment in the inflammation process. CCL5 is a potent chemoattractant for a number of different cell types, particularly eosinophils, basophils, and mononuclear cells. CCL5 is a potential biomarker for several diseases, for example, it can be useful for indicating the magnitude of asthmatic airway inflammation. In addition to being an important biomarker, CCL5 is studied for its interaction with its receptor, CCR5, in human immunodeficiency virus (HIV) research.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
For the detection of three biomarkers in complex sample matrices, the AlphaLISA and Electrochemiluminescent (ECL) assay technologies were shown to have similar: Assay windows (linear dynamic range), Lower and upper detection limit, Intra-and inter-assay precision (lower % CV) The advantages of using AlphaLISA over ECL are: Shorter total assay duration No wash steps No shaking Lower sample volume requirement for equivalent performance Less expensive instrument and plates required
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.