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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
CCL1 is Chemokine (C-C motif) ligand 1, also known as SCYA1, I-309, TCA3, P500, SISe. It is a 15 - 16 kD cytokine that belongs to a family inflammatory cytokines known as chemokines and is secreted by activated T cells. It attracts monocytes, NK cells, immature B cells, and dendritic cells by binding to the chemokine (C-C motif) receptor 8. This chemokine superfamily is involved in immunoregulatory and inflammatory processes.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
Protocol for AlphaLISA human CCL1 Detection Kit