TNFα (bovine) AlphaLISA Detection Kit, 100 Assay Points

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compati
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assayi>

TNFα is a polypeptide hormone mainly produced by activated macrophages and T cells. It is one of the most powerful mediators of inflammation, either playing a beneficial or deleterious role in disease progression. In particular, TNFα plays a clinically-relevant role as a mediator of immune responses associated to mastitis, endotoxic shock and bovine tuberculosis caused by Mycoplasma bovis. The present kit permits detection of bovine TNFα (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.