For research use only. Not for use in diagnostic procedures.
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IL-6 is a cytokine produced mainly T cells and macrophages. Levels of IL-6 have been reported to be increased in a variety of infectious diseases such as bovine diarrhea and tuberculosis. Also, levels of IL-6 have been shown to be increased following vaccination, sometimes correlating with antibody titers.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|