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IFNγ is mainly produced by activated T lymphocytes. Although by itself it has limited direct inflammatory effects, IFNγ is a major macrophage-activating factor, increasing TNFα and IL-1α production and thus contributing to activate acquired immunity to disease and passive immunity to vaccination. In particular, IFNγ has been associated to immune responses against bovine tuberculosis caused by Mycoplasma bovis. The present kit permits detection of bovine IFNγ (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|