PerkinElmer
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IFN-γ (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IFNγ in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL535HV
100 assay points
645.00 USD
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AL535C
500 assay points
2060.00 USD
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AL535F
5,000 assay points
10300.00 USD
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Detail Information

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

IFNγ is mainly produced by activated T lymphocytes. Although by itself it has limited direct inflammatory effects, IFNγ is a major macrophage-activating factor, increasing TNFα and IL-1α production and thus contributing to activate acquired immunity to disease and passive immunity to vaccination. In particular, IFNγ has been associated to immune responses against bovine tuberculosis caused by Mycoplasma bovis. The present kit permits detection of bovine IFNγ (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IFNγ
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Target Species Bovine
Therapeutic Area Inflammation
Unit Size 100 assay points