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BACE-1 (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
100 Assay Points
542.00 USD
500 Assay Points
1797.00 USD
5,000 Assay Points
11900.00 USD
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  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Beta-secretase 1 (BACE-1) is an aspartic protease encoded by the BACE-1 gene that is expressed ubiquitously throughout the body. BACE-1 has been identified as a necessary enzyme that functions with gamma-secretase to process of the amyloid precursor protein into the amyloid beta peptide, where the BACE-1 enzyme is responsible for the generation of the variable sizes of the peptide, particularly amyloid beta 1-40 and 1-42. As such, BACE1 is thought to be a valuable biomarker for Alzheimer’s disease, especially in the CSF. This AlphaLISA kit allows for the detection and quantification of BACE-1 in human serum, CSF, cell culture media and cell lysates.


Assay Target BACE-1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Cancer
Unit Size 5,000 Assay Points
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AlphaLISA Human BACE-1 Detection kit AL3033

This manual explains how to run the AlphaLISA no-wash human BACE-1 detection assay.

PDF 560 KB