PerkinElmer
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AST (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for human AST enables the quantitative determination of human aspartate transaminase (AST) in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Unit Size
List Price
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AL3078HV
100 Assay Points
912.00 USD
 
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AL3078C
500 Assay Points
2190.00 USD
 
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AL3078F
5,000 Assay Points
17600.00 USD
 
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is an important enzyme in amino acid metabolism. AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate. AST is found in metabolically active organgs and issues, such as the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Clinically, serum levels of AST and ALT (alanine transaminase) and their ratio (AST/ALT ratio) are used as biomarkers to monitor liver health. This kit has been designed for the detection of human AST in serum and cell culture media.

Specifications

Assay Target AST
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 5,000 Assay Points
Resources, Events & More
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

PDF 1 MB

Brochure

Technical Note

Assessing AST Released in a Cell Culture Model of Liver Toxicity Using AlphaLISA

Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is an important enzyme in amino acid metabolism. AST catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. The enzyme is found in metabolically active organs and tissues, such as the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Inflamed or injured liver cells leak higher than normal amounts of AST, and other enzymes, resulting in elevated levels found in the bloodstream. A blood test for AST is commonly used as one measure in the detection of liver damage.

In this technical note we demonstrate here the utility and benefits of using AlphaLISA assays for identifying and quantifying levels of AST protein present in cellular lysate and released into the supernatant from a human hepatoma cell line. The data illustrate the benefits of using AlphaLISA assays as a fast, powerful, homogeneous platform for screening modulators of AST expression and release in cultured human cells. The technology has a number of distinct advantages including high signal to background, wide dynamic range, and an extremely simple, straight-forward, no-wash protocol. Download our technical note to learn more.

PDF 1 MB