PEG IgM AlphaLISA Detection Kit, 500 Assay Points

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

The conjugation of small proteins, peptides, and oligonucleotides with polyethylene glycol (PEG), or PEGylation, has been shown to improve the pharmacokinetics of biological products by preventing their degradation, as well as improve tissue biodistribution and reducing immunogenicity and toxicity. However, there is an increasing body of data showing that PEG-conjugated compounds elicit IgG and IgM responses and anti-PEG antibodies also occur in 25% of healthy individuals. The presence of circulating anti-PEG antibodies may compromise the immunogenicity and limit the efficacy of therapeutic pegylated compounds. This AlphaLISA kit is designed to accurately measure levels of IgM anti-PEG antibodies in cell culture media, serum, and plasma.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.