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Angiopoietin 2 (Ang-2; also ANGPT2) is a glycoprotein which contains 478 amino acids when mature. It is a naturally occurring antagonist for both Angiopoietin 1 and TIE2, and expressed only at the sites of vascular remodeling; similar to Angiopoietin 1. Angiopoietin 2, unlike Angiopoietin 1, promotes cell death and disrupts vascularization. But when it is produced in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. This kit has been designed for the detection of Human Angiopoietin 2 in serum and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual explains how to run the AlphaLISA no-wash human ANGPT2 detection assay.