PerkinElmer
check quantity

ANGPT2 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human ANGPT2 enables the quantitative determination of human Angiopoietin 2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

You successfully added item(s) to your cart

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL3015HV
100 Assay Points
600.00 USD
more
AL3015C
500 Assay Points
2596.00 USD
more
AL3015F
5,000 Assay Points
18000.00 USD
more
Buy Now

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Angiopoietin 2 (Ang-2; also ANGPT2) is a glycoprotein which contains 478 amino acids when mature. It is a naturally occurring antagonist for both Angiopoietin 1 and TIE2, and expressed only at the sites of vascular remodeling; similar to Angiopoietin 1. Angiopoietin 2, unlike Angiopoietin 1, promotes cell death and disrupts vascularization. But when it is produced in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. This kit has been designed for the detection of Human Angiopoietin 2 in serum and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target ANGPT2
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Angiogenesis
Unit Size 5,000 Assay Points
Resources, Events & More
  • All

Brochure

Manual

AlphaLISA Human ANGPT2 Detection Kit AL3015

This manual explains how to run the AlphaLISA no-wash human ANGPT2 detection assay.

PDF 545 KB