PerkinElmer
check quantity

AlphaLISA SARS-CoV2 ACE2/S1 Binding Kit, 500 Assay Points

The AlphaLISA® SARS-CoV2 ACE2/S1 Binding Kit is a protein-protein interaction assay for ACE2-Spike inhibitor selection in biochemical format using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
AL3146C
500 Assay Points
2659.00 USD
 
more
AL3146F
5,000 Assay Points
13100.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

Angiotensin-converting enzyme 2 (ACE2) is a human cell surface receptor that has been identified as the main point of entry for SARS-CoV-2 virions, which match and bind ACE2 with their own viral Spike protein S1 subunits. This interaction is therefore a key target in COVID-19 therapeutic research, as its inhibitors may yield a protective effect by preventing SARS-CoV-2 entry into cells. For this reason, protein-protein interaction assays suitable for ACE2-Spike inhibitor screening are relevant to current research.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume

The AlphaLISA detection of SARS-CoV-2 S1/ACE2 binding uses acceptor beads coupled to human ACE2 protein and Streptavidin-coated donor beads to capture the biotinylated S1-protein. Donor beads and acceptor beads come into proximity through S1 binding to ACE2. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp emission at 615 nm (Figure 1).This assay can facilitate the screening of inhibitors of S1/ACE2 binding. A competitor of the interaction will disrupt the S1/ACE2 binding leading to a decrease of the AlphaLISA Signal.

Specifications

Assay Target SARS-CoV2 ACE2/S1
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Virology
Unit Size 500 Assay Points
Resources, Events & More
  • All

Application Note

Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

Alpha technology offers a simple, straight forward workflow. No wash needed!

  1. Add sample
  2. Add Acceptor bead mix; incubate 1 hr
  3. Add Donor beads; incubate 30 mins
  4. Read on Alpha-enable microplate reader

Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

PDF 1 MB

Poster

All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures

Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.

PDF 288 KB
AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

PDF 273 KB