Pre-formulated buffer that can be used in histone H3 peptide-based epigenetic assays.
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For research use only. Not for use in diagnostic procedures.
Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. This buffer prevents the non-specific binding of the highly-charged histone H3 (1-21) peptide to Donor and Acceptor beads in AlphaLISA epigenetic assays. Validated with Acceptor beads anti-H3K9ac (AL114), anti-H3K4me1-2 (AL116) and anti-H3K9me2 (AL117). It is used at the detection step for the dilution of Acceptor and Donor beads, not for the enzymatic reaction step. This buffer is not recommended for assays using full length histone proteins or nucleosomes.
Formulation: proprietary
| Automation Compatible | Yes |
|---|---|
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA |
| Shipping Condition | Blue Ice |
| Unit Size | 10 mL |
The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
Anti-mark antibodies coupled to AlphaLISA Acceptor beads or labeled with LANCE Ultra europium chelate were used for the successful optimization of robust and, sensitive epigenetic assays using histone H3-derived peptides as substrates.
In eukaryotes, the covalent modification of histones has a crucial role in chromatin architecture and plays an important part in a plethora of cellular processes, from chromatinre modeling and transcriptional regulation, to DNA repair and cell cycle control.
Covalen modification of DNA through methylation is catalyzed by specific DNA methyltransferases (DNMTs).
The AlphaLISA technology allows performing no-wash homogeneous proximity immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of anepigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate.
The AlphaLISA technology allows performing no-wash homogeneous proximity immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of anepigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate.
