For research use only. Not for use in diagnostic procedures.
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr951) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HUVEC cells were cultured to confluence in T175 flasks in 2% FBS medium, starved for 2 hours, then treated with 100 ng/mL rhVEGF for 5 minutes and lysed in 4 mL of 1X SureFire Ultra Lysis buffer.
Assay Target | VEGFR2 |
---|---|
Assay Target Class | Phosphoprotein |
Automation Compatible | Yes |
Detection Method | Alpha |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Unit Size | Vial |