Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.
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For research use only. Not for use in diagnostic procedures.
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-VEGF Receptor 2 (Tyr1175) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HUVEC cells grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.
| Assay Target | VEGFR2 |
|---|---|
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |