For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total Ubiquitin Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, PC3 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 25 nM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 120 min and lysed in 4 mL of 1X SureFire Ultra Lysis Buffer.
Assay Target | Ubiquitin |
---|---|
Assay Technology | Alpha |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Target Species | Human |
Therapeutic Area | Central Nervous System |