For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Ubiquitin is a highly conserved regulatory protein found in most tissues of eukaryotic organism. Phosphorylation of ubiquitin is a key step in the process of mitochondria quality control through the Parkin/PINK-1 interaction. Parkin is a ubiquitin ligase E3 that conjugates ubiquitin to impaired-mitochondrial proteins, leading to organelle degradation. PINK1 is a Ser/Thr kinase that accumulates only on impaired mitochondria and phosphorylates two substrates, the ubiquitin-like domain of Parkin and ubiquitin chains. The amplified Parkin/PINK-1 dependent ubiquitin functions act as a signal for mitophagy. Dysregulation of this signaling is linked to neurodegenerative diseases such as Parkinson's Disease.
The AlphaLISA SureFire Ultra Human Total Ubiquitin assay is a sandwich immunoassay for the quantitative detection of total ubiqiutin in cellular lysates, using Alpha Technology.
Assay Target | Ubiquitin |
---|---|
Assay Technology | Alpha |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Target Species | Human |
Therapeutic Area | Central Nervous System |
Unit Size | 50,000 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...