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AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

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Unit Size
List Price
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ALSU-PST3-A-HV
100 assay points
489.00 USD
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ALSU-PST3-A500
500 assay points
1653.00 USD
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ALSU-PST3-A10K
10,000 assay points
9100.00 USD
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ALSU-PST3-A50K
50,000 assay points
28400.00 USD
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Detail Information

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra p-STAT3 (Tyr705) assay, donor beads are coated with streptavidin to capture one of the antibodies used in the assay, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, leading to signal generation. The intensity of the light emission is directly proportional to the amount of phosphoprotein present in the sample, making the AlphaLISA SureFire Ultra an excellent immunoassay for quantitative detection of phospho-STAT3 in cellular lysates.

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) kits are compatible with cell and tissue lysates, antibody modulators, and biotherapeutic antibodies. These kits can be used for cellular kinase assays, receptor activation studies, and screening.

Specifications

Assay Pathway JAK/STAT
Assay Target STAT3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Quantity in a Package Amount 100.0 Units
Shipping Condition Blue Ice
Target Species Human
Unit Size 100 assay points