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In the AlphaLISA SureFire Ultra p-STAT3 (Tyr705) assay, donor beads are coated with streptavidin to capture one of the antibodies used in the assay, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, leading to signal generation. The intensity of the light emission is directly proportional to the amount of phosphoprotein present in the sample, making the AlphaLISA SureFire Ultra an excellent immunoassay for quantitative detection of phospho-STAT3 in cellular lysates.
AlphaLISA SureFire Ultra p-STAT3 (Tyr705) kits are compatible with cell and tissue lysates, antibody modulators, and biotherapeutic antibodies. These kits can be used for cellular kinase assays, receptor activation studies, and screening.
|Assay Target Class||Phosphoprotein|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Quantity in a Package Amount||10000.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 assay points|