The AlphaLISA® SureFire® Ultra™ total SMAD3 assay is a sandwich immunoassay for quantitative detection of SMAD3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.
For research use only. Not for use in diagnostic procedures.
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The AlphaLISA SureFire Ultra total SMAD3 assay is a sandwich immunoassay for quantitative detection of SMAD3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.
The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume. The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume. The 50,000 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
In the AlphaLISA SureFire Ultra assay, Donor Beads are coated with streptavidin to capture one of the, which is biotinylated. Acceptor Beads are coated with a proprietary CaptSureTM agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
|Assay Target Class||Protein|
|Assay Technology||AlphaLISA SureFire Ultra|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|