For research use only. Not for use in diagnostic procedures.
This control lysate is intended for use with the AlphaLISA SureFire Ultra total SMAD2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, RAW 264.7 cells were cultured to confluence in T175 flasks in 10% FBS-containing medium, then treated with 10 ng/mL rhTGFβ for 90min and lysed in 6 mL of 1X SureFire Ultra Lysis buffer.
Assay Pathway | TGFß/SMAD |
---|---|
Assay Target | Smad2 |
Assay Target Class | Protein |
Automation Compatible | Yes |
Detection Method | Alpha |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Therapeutic Area | Metabolic |
Unit Size | Vial |