For research use only. Not for use in diagnostic procedures.
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The AlphaLISA SureFire Ultra total SMAD1 assay is a sandwich immunoassay for quantitative detection of SMAD1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.
The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume. The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume. The 50,000 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
In the AlphaLISA SureFire Ultra assay, Donor Beads are coated with streptavidin to capture one of the, which is biotinylated. Acceptor Beads are coated with a proprietary CaptSureTM agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
|Assay Target Class||Protein|
|Assay Technology||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||50,000 assay points|