For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate Jurkat cells were cultured in T175 flask to 106 cells/mL in 10% FBS containing medium, then treated with 11 mM H2O2 and 50 nM Calyculin A for 10 minutes and lysed at 3.2X106 cells/mL with 1X SureFire Ultra Lysis Buffer.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|