For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-Ribosomal Protein S6 (Ser235/236) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate MCF7 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, starved for 2 hours, then treated with 500 μg/mL insulin for 60 minutes and lysed in 50 mL of 1X SureFire Ultra Lysis buffer.
Assay Target | Ribosomal Protein S6 |
---|---|
Assay Technology | Alpha |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |