Extra control lysate for the AlphaLISA® SureFire® Ultra™ Phospho-PLC G2 (Tyr759) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr759) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr759) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106 cells/mL with 5X SureFire Ultra Lysis buffer.
Application |
Protein Detection
|
---|---|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Unit Size |
1 Vial
|