For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217), or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106cells/mL with 5X SureFire Ultra Lysis buffer.
| Assay Target | PLC G2 |
|---|---|
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Autoimmunity, Central Nervous System, Oncology, Inflammation |
