Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-NFκB (Ser536) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-NFκB assay kit, but can also be ordered separately.
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For research use only. Not for use in diagnostic procedures.
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-NFκB p65 (Ser536) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium for 3 days, then treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.
| Assay Target | NFκB |
|---|---|
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |