For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Total SLP76 Detection Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were cultured in T175 flask to 1 x 106 cells/mL in 10% FBS containing medium, then treated with 11 mM H2O2 and 50 nM Calyculin A in HBSS for 10 minutes and lysed at 3.2 x 106 cells/mL with 1X SureFire Ultra Lysis Buffer.
Assay Target | SLP76 |
---|---|
Assay Technology | Alpha |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Target Species | Human, Mouse |
Therapeutic Area | Autoimmunity, Oncology, Immuno-oncology |