For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-MLKL (Ser345) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 20 μM Z-VAD, 100 nM SM-164 and 50 ng/mL TNFα for 4 hours at 37°C. Cells were lysed in 5 mL 1X SureFire Ultra Lysis buffer.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Neuroinflammation, Oncology, Inflammation|
|Unit Size||1 vial|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
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