For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-MLKL (Ser345) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 20 μM Z-VAD, 100 nM SM-164 and 50 ng/mL TNFα for 4 hours at 37°C. Cells were lysed in 5 mL 1X SureFire Ultra Lysis buffer.
| Assay Target | MLKL |
|---|---|
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Neuroinflammation, Oncology, Inflammation |
| Unit Size | 1 vial |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...
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