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AlphaLISA SureFire Ultra Phospho-MLKL (Ser358), Lysate

Extra control lysate for the AlphaLISA® SureFire® Ultra Phospho-MLKL (Ser358) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) assay kit, but can also be ordered separately.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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ALSU-PMLKL-B-L
26.00 USD
 
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Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in T175 flasks to 106 cells/mL in RPMI containing 10% FBS. Cells were treated with 20 μM Z-VAD for 30 minutes at 37°C, and then treated with 100 nM SM-164 and 20 ng/mL TNFα for 4 hours at 37°C. Total stimulus time 4.5 hours. Cells were then washed with HBSS and lysed in 5 mL 1X SureFire Ultra Lysis buffer.

Specifications

Assay Target MLKL
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice