The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
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For research use only. Not for use in diagnostic procedures.
The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology.
In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA® SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
| Assay Pathway | MAPK |
|---|---|
| Assay Target | MEK1 |
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Second Messenger Release | Protein phosphorylation |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Unit Size | 10,000 Assay Points |
