The AlphaLISA® SureFire® Ultra™ total Insulin Receptor β assay is a sandwich immunoassay for quantitative detection of Akt1/2/3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Insulin Receptor β assay.
For research use only. Not for use in diagnostic procedures.
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NULL OR EMPTY CART
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra™ kits are compatible with:
Alpha SureFire kits can be used for:
|Assay Target||Insulin Rec. β|
|Assay Target Class||Protein|
|Assay Technology||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|