For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-IkBα (Ser32/36) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 5 minutes at 37°C and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Target Species||Human, Mouse|
|Therapeutic Area||Autoimmunity, Oncology, Immuno-oncology|