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AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227), Control Lysate

Extra control lysate for the AlphaLISA® SureFire® Ultra Phospho-RIP3 (Ser227) (Human) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Ser227) (Human) assay kit, but can also be ordered separately.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were seeded at 800 K/mL and grown overnight in T175 flasks in 50 mL of 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 7 hours at 37°C. Cells were harvested, washed with HBSS and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.


Assay Target RIP3
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Autoimmunity, Central Nervous System, Neuroinflammation