For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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NULL OR EMPTY CART
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were seeded at 800 K/mL and grown overnight in T175 flasks in 50 mL of 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 7 hours at 37°C. Cells were harvested, washed with HBSS and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Autoimmunity, Central Nervous System, Neuroinflammation|