For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were seeded at 800 K/mL and grown overnight in T175 flasks in 50 mL of 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 7 hours at 37°C. Cells were harvested, washed with HBSS and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
| Assay Target | RIP3 |
|---|---|
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Therapeutic Area | Autoimmunity, Central Nervous System, Neuroinflammation |
OptiPlate-384, White Opaque 384-well Microplate, Case 50
List Price : USD344.0
AlphaPlate-384, Light gray, untreated, Case of 50
List Price : USD635.0
List Price : USD79.0
