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AlphaLISA SureFire Ultra Human and Mouse Phospho-Histone H3 (Ser10/Thr11) Detection Kit, HV

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-Histone H3 (Ser10/Thr11) assay is a sandwich immunoassay for quantitative detection of phospho-Histone H3 (Ser10/Thr11) in cellular lysates using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
500 Assay Points
1846.00 USD
10,000 Assay Points
11400.00 USD
50,000 Assay Points
32400.00 USD
100 Assay Points
640.00 USD
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Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones and is phosphorylated during mitosis and meiosis. Aurora kinase B is directly responsible for mitotic Histone H3 Ser10 phosphorylation. Aurora kinase A is amplified in human cancers, with its overexpression in human tumors correlating with a poor prognosis and genomic instability. Histone H3 is implicated in glioblastomas, ovarian carcinomas, and hepatocellular carcinomas.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-Histone H3 (Ser10/Thr11) assay is a sandwich immunoassay for the quantitative detection of phospho-histone H3 (Ser10/Thr11) in cellular lysates, using Alpha Technology.


  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


Assay Target Histone H3
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Target Species Human, Mouse
Therapeutic Area Oncology
Unit Size 100 Assay Points
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Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.



Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

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