The AlphaLISA® SureFire® Ultra™ total CDK2 assay is a sandwich immunoassay for quantitative detection of CDK2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® SureFire® Ultra™ total CDK2 assay is a sandwich immunoassay for quantitative detection of total CDK2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.
Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
50000
|
Assay Target Class |
Phospho-protein
Protein
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Species |
Human
|
Therapeutic Area |
Oncology
|
Unit Size |
50,000 Assay Points
|