For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-CD3 ζ (Tyr83) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were seeded at 500 K/mL and grown overnight in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 3.5 X 106 cells/mL and treated with 44 mM H2O2 for 10 minutes. Cells were lysed with SureFire Ultra Lysis Buffer.
| Assay Target | CD3 ζ |
|---|---|
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Therapeutic Area | Autoimmunity, Oncology, Immuno-oncology |
OptiPlate-384, White Opaque 384-well Microplate, Case 50
List Price : USD344.0
AlphaPlate-384, Light gray, untreated, Case of 50
List Price : USD635.0
List Price : USD79.0
