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AlphaLISA SureFire Ultra Human Phospho-CD3 ζ (Tyr142), Control Lysate

Extra control lysate for the AlphaLISA® SureFire® Ultra Phospho-CD3 ζ (Tyr142) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-CD3 ζ (Tyr142) assay kit, but can also be ordered separately.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-CD3 ζ (Tyr142) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were seeded at 500 K/mL and grown overnight in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 3.5 X 106 cells/mL and treated with 44 mM H2O2 for 10 minutes. Cells were lysed with SureFire Ultra Lysis Buffer.


Assay Target CD3 ζ
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Autoimmunity, Oncology, Immuno-oncology