For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Total ß-Catenin Detection Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 1 μg/mL PMA and 50 nM Calyculin A for 4 hours at 37°C. Cells were lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
Assay Target | ß-Catenin |
---|---|
Assay Technology | Alpha |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Target Species | Human, Mouse |
Therapeutic Area | Central Nervous System, Oncology |