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AlphaLISA SureFire Ultra Human Total BRD4 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Total BRD4 assay is a sandwich immunoassay for quantitative detection of total BRD4 in cellular lysates using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
ALSU-TBRD4-A500
500 Assay Points
1648.00 USD
 
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ALSU-TBRD4-A10K
10,000 Assay Points
10200.00 USD
 
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ALSU-TBRD4-A50K
50,000 Assay Points
29000.00 USD
 
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ALSU-TBRD4-A-HV
100 Assay Points
571.00 USD
 
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Overview

Bromodomain-containing protein 4 (BRD4) is a member of the Bromodomains and Extraterminal (BET) family, consisting of BRD2, BRD3, and BRDT. BRD4 is a transcription regulator consisting of two bromodomains which bind acetylated lysine residues on proteins, such as histones. In association with Cyclin T1 and CDK9, BRD4 forms a complex called P-TEFb which controls gene transcription. BRD4 is also involved in DNA damage response, embryogenesis, and cancer development. Inhibiting BRD4 is a strategy for treating cancer, and more recently targeting BRD4 with PROTAC molecules has emerged as a novel therapeutic approach.

The AlphaLISA SureFire Human Total BRD4 Detection Kit is a sandwich immunoassay for the quantitative detection of total BRD4 in cellular lysates, using Alpha Technology.

Formats

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Points (200-50,000) 50000 Assay Points
Assay Target BRD4
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Oncology
Unit Size 50,000 Assay Points
Resources, Events & More
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

PDF 4 MB