PerkinElmer
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AlphaLISA SureFire Ultra Total Akt1 Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra total Akt1 is a sandwich immunoassay for quantitative detection of Akt1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
ALSU-TAKT1-B500
500 assay points
1791.00 USD
 
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ALSU-TAKT1-B10K
10,000 assay points
9900.00 USD
 
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ALSU-TAKT1-B50K
50,000 assay points
32000.00 USD
 
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ALSU-TAKT1-B-HV
100 assay points
530.00 USD
 
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Overview

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

 

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1
Assay Target Class Protein
Assay Technology AlphaLISA SureFire Ultra
Automation Compatible Yes
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Therapeutic Area Central Nervous System, Cardiovascular, Metabolic
Unit Size 100 assay points
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Brochure

GPCRs: The Pathway to Discovery

G protein coupled-receptors (GPCRs) are one of the most intensively studied drug targets, with up to one-third of all marketed therapeutics acting by binding to GPCRs. Therapies that target GPCRs include both agonist and antagonists that are used in the treatment of disease in nearly every major organ system and hold significance in several therapeutic areas, such as cardiovascular diseases, neurosciences, respiratory diseases, metabolic disorders, oncology, and rare diseases.

We know the importance of furthering GPCR research - and we answer the call with application notes, white papers, and end-to-end solutions including reagents, instruments, and tools for all aspects of research into today’s most promising drug target.

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