For research use only. Not for use in diagnostic procedures.
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NULL OR EMPTY CART
This positve control lysate is prepared from HEK293 cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 20% FBS for 15 minutes and lysed with 1X SureFire Ultra Lysis buffer.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System, Cardiovascular, Metabolic|
|Unit Size||1 vial|
In this application note, we demonstrate an efficient cell-based workflow for the assessment of EGF treatment effects in a cellular model of human skin cancer.
Treatment effects on several intracellular signaling pathways were examined using PerkinElmer’s homogeneous, no-wash AlphaLISA® SureFire® Ultra assays. To determine concurrent time-dependent effects of different EGF concentrations on cellular health and proliferation, ATP concentrations were assessed with ATPlite™ 1step luminescence assay and cultures were fluorescently labeled, imaged and analyzed using the Operetta CLS™ high-content analysis system.