For research use only. Not for use in diagnostic procedures.
This positve control lysate is prepared from HEK293 cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 20% FBS for 15 minutes and lysed with 1X SureFire Ultra Lysis buffer.
| Assay Target | Akt1/2/3 |
|---|---|
| Assay Target Class | Protein |
| Detection Method | Alpha |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Central Nervous System, Cardiovascular, Metabolic |
| Unit Size | 1 vial |
In this application note, we demonstrate an efficient cell-based workflow for the assessment of EGF treatment effects in a cellular model of human skin cancer.
Treatment effects on several intracellular signaling pathways were examined using PerkinElmer’s homogeneous, no-wash AlphaLISA® SureFire® Ultra assays. To determine concurrent time-dependent effects of different EGF concentrations on cellular health and proliferation, ATP concentrations were assessed with ATPlite™ 1step luminescence assay and cultures were fluorescently labeled, imaged and analyzed using the Operetta CLS™ high-content analysis system.
Total AKT1/2/3 AlphaLISA SureFire Ultra Detection Kit, 10,000 Assay Points
List Price : USD11400.0
