For research use only. Not for use in diagnostic procedures.
This positve control lysate is prepared from HEK293 cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 20% FBS for 15 minutes and lysed with 1X SureFire Ultra Lysis buffer.
Assay Target | Akt1/2/3 |
---|---|
Assay Target Class | Protein |
Detection Method | Alpha |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Therapeutic Area | Central Nervous System, Cardiovascular, Metabolic |
Unit Size | 1 vial |
In this application note, we demonstrate an efficient cell-based workflow for the assessment of EGF treatment effects in a cellular model of human skin cancer.
Treatment effects on several intracellular signaling pathways were examined using PerkinElmer’s homogeneous, no-wash AlphaLISA ...